CHAPTER 4 PRINCIPLES AND PROCESSES OF BIOTECHNOLOGY - CLASS 12 BIOLOGY BOTANY - 1 MARK QUESTIONS - ONLINE TEST

ப்ளஸ் டூ படிக்கும் மாணவர்களுக்கு இந்தத் தேர்வு பயனுள்ளதாக இருக்கும். இந்த யூ ட்யூப் தளத்தில் வரும் ஆடியோவை ப்ளே செய்து மீண்டும் மீண்டும் கேளுங்கள். ஆடியோவை கேட்கும் பொழுதே கீழே உள்ள கேள்வி பதில்களை SCROLL செய்து உற்று நோக்குங்கள். பிறகு கேள்விகளில் உள்ள சரியான விடையை தேர்ந்தெடுத்து கடைசியாக SUBMIT செய்யுங்கள். நீங்கள் எடுத்த மதிப்பெண் வரும் அந்த மதிப்பெண்ணை கீழே உள்ள கமெண்ட் பாக்ஸ் இல் பதிவு செய்யுங்கள். மதிப்பெண் குறைந்தால் மீண்டும் ஒருமுறை முயற்சி செய்யுங்கள்... வாழ்த்துக்கள் மாணவர்களே...அன்புடன் - K.K.D.

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1.

Restriction enzymes are

a. Not always required in genetic engineering

b. Essential tools in genetic engineering

c. Nucleases that cleave DNA at specific sites

d. both b and c

2.

Plasmids are

a. circular protein molecules

b. required by bacteria

c. tiny bacteria

d. confer resistance to antibiotics

3.

EcoRI cleaves DNA at

a. AGGGTT

b. GTATATC

c. GAATTC

d. TATAGC

4.

Genetic engineering is

a. making artificial genes.

b. hybridization of DNA of one organism to that of the others.

c. production of alcohol by using micro organisms.

d. making artificial limbs, diagnostic instruments such as ECG, EEG etc.,

5.

Consider the following statements :
➡️I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
➡️II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
➡️III. Restriction enzymes belongs to a class of enzymes called nucleases./ Choose the correct option regarding above statements

a. I & II

b. I & III

c. II & III

d. I,II & III

6.

The process of recombinant DNA technology has the following steps :
➡️I. amplication of the gene
➡️II. Insertion of recombinant DNA into the host cells
➡️III. Cutting of DNA at specific location using restriction enzyme .
➡️IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.

a. II, III, IV, I

b. IV, II, III, I

c. I, II, III, IV

d. IV, III, I, II

7.

Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction enzymes?

5' CGTTCG 3' 3' ATCGTA 5'

5' GATATG 3' 3' CTACTA 5'

5'GAATTC 3' 3' CTTAAG 5'

5' CACGTA 3' 3' CTCAGT 5'

8.

pBR 322, BR stands for

a. Plasmid Bacterial Recombination

b. Plasmid Bacterial Replication

c. Plasmid Boliver and Rodriguez

d. Plasmid Baltimore and Rodriguez

9.

Which of the following one is used as a Biosensors?

a. Electrophoresis

b. Bioreactors

c. Vectors

d. Electroporation

10.

Match the following :
Column A :
❇️ [1] Exonuclease
❇️ [2] Endonuclease
❇️[3] Alkaline
❇️[4] Ligase
Column B :
📍 [a] add or remove phosphate
📍 [b] binding the DNA fragments
📍 [c] cut the DNA at terminus
📍 [d] cut the DNA at middle

1 (a), 2(b), 3 (c), 4 (d)

1 (c), 2(d), 3 (b), 4 (a)

1 (a), 2(c), 3 (b), 4 (d)

1 (c), 2(d), 3 (a), 4 (b)

11.

In which techniques Ethidium Bromide is used?

a. Southern Blotting techniques

b. Western Blotting techniques

c. Polymerase Chain Reaction

d. Agrose Gel Electroporosis

12.

➡️Assertion : Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
➡️Reason: A gene incorporated in the bacterial chromosomal genome gets atomatically transferred to the cross with which bacterium is associated.

a) Both assertion and reason are true. But reason is correct explanation of assertion.

b) Both assertion and reason are true. But reason is not correct explanation of assertion.

c) Assertion is true, but reason is false.

d) Assertion is false, but reason is true.

e) Both assertion and reason are false.

13.

Which one of the following is not correct statement.

a) Ti plasmid causes the bunchy top disease

b) Multiple cloning site is known as Polylinker

c) Non viral method transfection of Nucleic acid in cell

d) Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

14.

An analysis of chromosomal DNA using the southern hybridisation technique does not use

a) Electrophoresis

b) Blotting

c) Autoradiography

d) Polymerase Chain Reaction

15.

An antibiotic gene in a vector usually helps in the selection of

a) Competent cells

b) Transformed cells

c) Recombinant cells

d) None of the above

16.

Some of the characteristics of Bt cotton are

a) Long fibre and resistant to aphids

b) Medium yield, long fibre and resistant to beetle pests

c) high yield and production of toxic protein crystals which kill dipteran pests.

d) High yield and resistant to ball worms

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